Speedily get sections as slim or thick as your software demands, devoid of stressing about losing or distorting beneficial tissue samples.
Megatome is really a vibrating microtome designed to segment a broad array of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables large-throughput tissue sectioning with uniform surface profile, as well as minimum tissue injury and data reduction.
Antibodies may possibly acquire months to diffuse by way of just a few millimeters of tissue, that has a steep labeling gradient from floor to Main.
Functioning the CLI straight from a Gradle endeavor will not be at this time supported. A distribution need to be made by means of gradlew :j2d-cli:distZip to make a zip file that contains almost everything required to operate.
SE takes advantage of a rotational electric powered subject to disperse hugely electromobile molecules (for example antibodies or surfactant micelles) during a porous sample without harming electrically charged structures throughout the tissue. This allows two-4 day clearing of intact organs,
venture to create an aggregated Javadoc. With this occasion, javadocTask ought to be established to allJavadoc in order that the right documentation is constructed before building the docset.
Megatome can be a novel microtome that allows for top-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with negligible tissue hurt and information decline.
eFLASH is actually a immediate tissue labeling strategy that allows for uniform total-organ staining in 20 rounds of labeling.
Fully delipidate complete mouse brains or comparably sized samples in only one day with SmartBatch+, or in a single megatomi.com week with our passive clearing package.
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Defend avoids the variability of hydrogel embedding and the data decline from PFA preservation, safeguarding specimens for various rounds of processing.
Leverage the Very clear+ tissue clearing process, coupled with eFLASH and patented stochastic electrotransport technologies, to swiftly obvious and label total organs. Critical highlights and capabilities involve:
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